Large language models remain vulnerable to jailbreak attacks, and single-layer defenses often trade security for usability. We present TRYLOCK, the first defense-in-depth architecture that combines four heterogeneous mechanisms across the inference stack: weight-level safety alignment via DPO, activation-level control via Representation Engineering (RepE) steering, adaptive steering strength selected by a lightweight sidecar classifier, and input canonicalization to neutralize encoding-based bypasses. On Mistral-7B-Instruct evaluated against a 249-prompt attack set spanning five attack families, TRYLOCK achieves 88.0% relative ASR reduction (46.5% to 5.6%), with each layer contributing unique coverage: RepE blocks 36% of attacks that bypass DPO alone, while canonicalization catches 14% of encoding attacks that evade both. We discover a non-monotonic steering phenomenon -- intermediate strength (alpha=1.0) degrades safety below baseline -- and provide mechanistic hypotheses explaining RepE-DPO interference. The adaptive sidecar reduces over-refusal from 60% to 48% while maintaining identical attack defense, demonstrating that security and usability need not be mutually exclusive. We release all components -- trained adapters, steering vectors, sidecar classifier, preference pairs, and complete evaluation methodology -- enabling full reproducibility.